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CHD4 Antibody [3F2/4]

Additional information

Catalog Number: 1108-MSM1
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1108-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1108-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1108-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CHD4 Antibody

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CHD4 Mouse Monoclonal Antibody (3F2/4).

CHD4 Antibody - Image 2

SDS-PAGE Analysis of Purified CHD4 Mouse Monoclonal Antibody (3F2/4). Confirmation of Purity and Integrity of Antibody.

CHD4 Antibody - Image 3

Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).

CHD4 Antibody - Image 4

Confocal immunofluorescence image of MCF-7 cells using CHD4 Mouse Monoclonal Antibody (3F2/4) stained in Green (CF488) and Phalloidin is used to label the membranes.

  • ELISA
  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Mouse
Clone
3F2/4
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Centrosome, Cytoplasm, Cytoskeleton, Microtubule organizing center, Nucleus
Gene Name
CHD4
Positive Control
HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.
Immunogen
Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
Key References
  • Sparmann et al. 2013. EMBO J. 32(11):1598-612.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1108
SwissProt
Q14839
Chromosome Location
12p13.31
Mol. Weight of Antigen
238kDa
Alternate Names
Chromodomain-helicase-DNA-binding protein 4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, ATP dependent helicase CHD4; Chromodomain helicase DNA binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi2 beta

Summary

CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

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