Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CHD4 Mouse Monoclonal Antibody (3F2/4).

SDS-PAGE Analysis Purified CHD4 Mouse Monoclonal Antibody (3F2/4). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).

Confocal immunofluorescence image of MCF-7 cells using CHD4 Mouse Monoclonal Antibody (3F2/4) stained in Green (CF488) and Phalloidin is used to label the membranes.

Printable Datasheet

CHD4 [3F2/4]

Name: CHD4
SKU: 1108-MSM1
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

Product Properties & Targets

Host

Mouse

Applications

ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Species Reactivity

Human, Mouse

Isotype

IgG2a

Cellular Localization

Centrosome, Cytoplasm, Cytoskeleton, Microtubule organizing center, Nucleus

Light Chain

Kappa

Gene Name

CHD4

Positive Control

HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.

Immunogen

Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.

Alternate Names

Chromodomain-helicase-DNA-binding protein 4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, ATP dependent helicase CHD4; Chromodomain helicase DNA binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi2 beta

Database Links

Entrez Gene ID

1108

Unigene

162233

SwissProt

Q14839

Additional Information

Clone

3F2/4

Human Gene Symbol

CHD4

Chromosome Location

12p13.31

Mol. Weight of Antigen

238kDa

Functions

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.

Known Applications & Suggested Dilutions

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Sparmann et al. 2013. EMBO J. 32(11):1598-612.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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