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CELA3A / Chymotrypsin like elastase 3A Antibody [CELA3A/12552]

Additional information

Catalog Number: 10136-MSM2
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
10136-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
10136-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
10136-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CELA3A Antibody in Immunohistochemistry (IHC (P))

CELA3A Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human pancreas stained with CELA3A Mouse Monoclonal Antibody (CELA3A/12552). Inset: PBS instead of primary antibody; secondary only negative control.

CELA3A Antibody in SDS-PAGE

CELA3A Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CELA3A Mouse Monoclonal Antibody (CELA3A/12552). Confirmation of Purity and Integrity of Antibody.

CELA3A Antibody in Western Blot (WB)

CELA3A Antibody in Western Blot (WB)

Western Blot Analysis of human pancreas lysate using CELA3A Mouse Monoclonal Antibody (CELA3A/12552).

CELA3A Antibody in Western Blot (WB)

CELA3A Antibody in Western Blot (WB)

Western blot analysis of skeletal muscle tissue lysates from guinea pig, rat, mouse, hamster and human using CELA3A Mouse Monoclonal Antibody (CELA3A/12552).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
CELA3A/12552
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm, Extracellular space
Gene Name
CELA3A
Positive Control
Human pancreas or pancreatic cancer.
Immunogen
Recombinant fragment (around aa50-250) of human CELA3A protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Efficient protease with alanine specificity but only little elastolytic activity.
Key References
  • Qiao, X., Qi, X., Xing, P., Liu, T., Hao, H., Yang, X., Jiang, B., Cui, M., & Su, X. (2023). Tandem Mass Tag-Based Proteomic Profiling Identifies Biomarkers in Drainage Fluid for Early Detection of Anastomotic Leakage after Rectal Cancer Resection. Journal of proteome research, 22(11), 3559–3569. https://doi.org/10.1021/acs.jproteome.3c00394
  • Xiao, Y., Zhang, B., Cloyd, J. M., Xu, G., Du, S., Mao, Y., & Pawlik, T. M. (2022). Gene signature and connectivity mapping to assist with drug prediction for pancreatic ductal adenocarcinoma. Surgical oncology, 44, 101849. https://doi.org/10.1016/j.suronc.2022.101849
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
10136
SwissProt
P09093
Chromosome Location
1p36.12
Mol. Weight of Antigen
29 kDa
Alternate Names
chymotrypsin-like elastase family member 3A, Chymotrypsin-like elastase family member 3A; Elastase IIIA; Elastase-3A; Protease E; ELA3A; ELA3; CELA3A, ELA3; ELA3A

Summary

Elastases belong to a subfamily of serine proteases that function to catalytically hydrolyze a wide variety of proteins. Elastase-3A, also known as ELA3A or ELA3, is a 270 amino acid protein that belongs to the elastase family and acts as an alanine-specific protease. Secreted as a zymogen from the pancreas, Elastase-3A functions as a digestive enzyme in the intestine that participates in metabolic degradation pathways by preferentially cleaving proteins that have alanine residues. In addition, Elastase-3A is thought to play a role in the metabolism and transport of cholesterol molecules in the intestine. Altered expression of Elastase-3A may be associated with pancreatic ductal carcinoma, a highly fatal cancer of the pancreatic ducts

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