Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Rabbit Polyclonal Antibody.

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Rabbit Polyclonal Antibody.

Printable Datasheet

CD34 (Hematopoietic Stem Cell & Endothelial Marker) [N/A]

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Summary

This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Product Properties & Targets

Host
Rabbit
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Rat
Research Areas
Hypoxia
Isotype
IgG
Cellular Localization
Membrane
Light Chain
Kappa
Gene Name
CD34
Positive Control
KG-1 cells. Human tonsil or angiosarcoma.
Immunogen
Recombinant full-length human HPCA1 protein
Alternate Names
Hematopoietic progenitor cell antigen CD34, Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Database Links

Entrez Gene ID
947
Unigene
374990
SwissProt
P28906

Additional Information

Clone
N/A
Human Gene Symbol
CD34
Chromosome Location
1q32.2
Mol. Weight of Antigen
90-110kDa

Functions

  • Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Known Applications & Suggested Dilutions

  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • Western Blot (1-2ug/ml)
  • Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Key References

  • Felshow DM et al. Blood 97:3768-3775 (2001).
  • Sato T et al. Blood 94:2548-2554 (1999).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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