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CD20 / MS4A1 (B-Cell Marker) Antibody [93-1B3]

In Stock
Catalog Number Formulation Size Price
931-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
931-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
931-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD20 / MS4A1 (B-Cell Marker) Antibody

SDS-PAGE Analysis of Purified CD20 Mouse Monoclonal Antibody (93-1B3). Confirmation of Integrity and Purity of Antibody.

CD20 Antibody in Western Blot (WB)

CD20 Antibody in Western Blot (WB)

Western blot analysis of Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine, Rabbit Intestine, Human Spleen, Mouse Spleen, Rat Spleen, Hamster Spleen, Guinea pig Spleen and Rabbit Spleen tissue lysates using CD20 Mouse Monoclonal Antibody (93-1B3).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 30-33kDa, which is identified as CD20 (Workshop V; Code CD20.4). It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence, Western Blot
Species Reactivity
Human
Research Areas
B Cell Markers, Hematopoietic Stem Cells
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
MS4A1
Positive Control
Daudi, Raji and U266 and human intestine, Spleen, lymphocytes. Lymph nodes and tonsils.
Immunogen
Stimulated human leukocytes
Alternate Names
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1 (MS4A1)

Database Links

Entrez Gene ID
931
SwissProt
P11836

Additional Information

Clone
93-1B3
Chromosome Location
11q12.2
Mol. Weight of Antigen
33-37kDa

Related Antibodies

View All MS4A1 Antibodies

Functions

  • B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602).

Key References

  • Cobbold, S. Et al., In leucocyte typing III (ed. McMichael A.J. et al.), Oxford University Press, 1987.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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