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CD14 (Monocyte / Macrophage Marker) Antibody [LPSR/7738]

In Stock
Catalog Number Formulation Size Price
929-MSM16-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
929-MSM16-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
929-MSM16-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human lymph node stained with CD14 Mouse Monoclonal Antibody (LPSR/7738). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human lymph node stained with CD14 Mouse Monoclonal Antibody (LPSR/7738). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with CD14 Mouse Monoclonal Antibody (LPSR/7738). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with CD14 Mouse Monoclonal Antibody (LPSR/7738). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis of Purified CD14 Mouse Monoclonal Antibody (LPSR/7738). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified CD14 Mouse Monoclonal Antibody (LPSR/7738). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, B Cell Markers, Dendritic Cell Marker, Hematopoietic Stem Cells, Infectious Disease, MAPK Signaling, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG2 / Kappa
Cellular Localization
Cell surface
Gene Name
CD14
Positive Control
A549|THP-1 cells and human monocytes. Lymph nodes and tonsils.
Immunogen
Recombinant fragment of human CD14 protein (around aa 1-200) (exact sequence is proprietary)
Alternate Names
Monocyte differentiation antigen CD14 | Monocyte differentiation antigen CD14, urinary form | Monocyte differentiation antigen CD14, membrane-bound form, Myeloid cell-specific leucine-rich glycoprotein, Lipopolysaccharide receptor; LPS-R; LPSR; Mo2; Monocyte Differentiation Antigen 14; Myeloid cell-specific leucine-rich glycoprotein

Database Links

Entrez Gene ID
929
SwissProt
P08571

Additional Information

Clone
LPSR/7738
Chromosome Location
5q31.1
Mol. Weight of Antigen
55kDa

Related Antibodies

View All CD14 Antibodies

Functions

  • Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655, PubMed:22265692). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).

Key References

  • Simmons, D.L., et al. 1989. Monocyte antigen CD14 is a phospholipid anchored membrane protein. Blood 73: 284-289.
  • Schumann, R.R. 1992. Function of lipopolysaccharide (LPS)-binding protein (LBP) and CD14, the receptor for LPS/LBP complexes: a short review. Res. Immunol. 143: 11-15.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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