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CD137L / 4-1BBL / TNFSF9 Antibody [CD137L/1547]

Additional information

Catalog Number: 8744-MSM1-B1
Reactivity:

Gene:

Host:

In Stock

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In Stock
Best Seller
Catalog Number Formulation Size Price
8744-MSM1-B1
Purified Ab conjugated to Biotin
0.5ml at 100ug/ml
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD137L / 4-1BBL / TNFSF9 Antibody

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD137L-Monospecific Mouse Monoclonal Antibody (CD137L/1547).

CD137L / 4-1BBL / TNFSF9 Antibody - Image 2

SDS-PAGE Analysis Purified CD137L-Monospecific Mouse Monoclonal Antibody (CD137L/1547). Confirmation of Purity and Integrity of Antibody.

CD137L / 4-1BBL / TNFSF9 Antibody - Image 3

Flow Cytometric Analysis of PFA-fixed HEK293 cells using CD137L-Monospecific Mouse Monoclonal Antibody (CD137L/1547) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Flow
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Clone
CD137L/1547
Isotype
IgG2a
Cellular Localization
Membrane
Gene Name
TNFSF9
Positive Control
Raji
Immunogen
Recombinant full-length human CD137L protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
Key References
  • Qian Y, et al. Med Oncol, 2015 Mar. PMID 25631633
  • Zhao S, et al. Am J Surg Pathol, 2013 Feb. PMID 23095505
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
8744
SwissProt
P41273
Chromosome Location
19p13.3
Mol. Weight of Antigen
27kDa
Alternate Names
Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BB ligand; 4-1BBL; CD137L; ILA ligand (TNF related); Ly63l; Tumor necrosis factor ligand 5A (TNLG5A); Tumor necrosis factor ligand superfamily member 9 (TNFSF9)

Summary

TNFSF9 / 4-1BBL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9 / 4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9 / 4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. It has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine is required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.

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