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  • HuProt™ Validated

CD103 / Integrin alpha E (T-Cell Lymphoma & Hairy Cell Leukemia Marker) Antibody [ITGAE/2474]

These antibodies are validated on HuProt™ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE

Additional information

Catalog Number: 3682-MSM4
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3682-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3682-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3682-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
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CD103 / Integrin alpha E (T-Cell Lymphoma & Hairy Cell Leukemia Marker) Antibody

Formalin-fixed, paraffin-embedded human Small Intestine stained with CD103 Mouse Monoclonal Antibody (ITGAE/2474).

CD103 / Integrin alpha E (T-Cell Lymphoma & Hairy Cell Leukemia Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Colon stained with CD103 Mouse Monoclonal Antibody (ITGAE/2474).

CD103 / Integrin alpha E (T-Cell Lymphoma & Hairy Cell Leukemia Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human Colon stained with CD103 Mouse Monoclonal Antibody (ITGAE/2474).

CD103 / Integrin alpha E (T-Cell Lymphoma & Hairy Cell Leukemia Marker) Antibody - Image 4

SDS-PAGE Analysis Purified CD103 Mouse Monoclonal Antibody (ITGAE/2474). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
ITGAE/2474
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Membrane
Gene Name
ITGAE
Positive Control
Colon or Stomach, Tonsil
Immunogen
Recombinant human ITGAE/CD103 protein fragment (aa 775-855) (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Reactivity:

, ,

Gene:

Host:

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Reactivity:

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
Key References
  • Morgan EA,Yu H,Pinkus JL,Pinkus GS. Immunohistochemical detection of hairy cell leukemia in paraffin sections using a highly effective CD103 rabbit monoclonal antibody. Am J Clin Pathol.2013 Feb;139(2):220-30.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3682
SwissProt
P38570
Chromosome Location
17p13
Mol. Weight of Antigen
150kDa
Alternate Names
Integrin alpha-E, HML-1 antigen, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen, CD103; Human mucosal lymphocyte antigen 1 (HML1 antigen); Integrin alpha E heavy chain; Integrin alpha E light chain; Integrin alpha E, epithelial-associated; Integrin alpha E1; Integrin alpha-IEL (intestinal intraepithelial lymphocytes); Integrin alpha M290; Integrin alpha-E heavy chain; Integrin, alpha E (ITGAE)

Summary

This MAb recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Antibody to CD103 is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).

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