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BRAF Antibody [BRAF/1626]

In Stock
Catalog Number Formulation Size Price
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes


The �BRAF �gene encodes a cytoplasmic serine-threonine kinase, which initiates the activation of the mitogen-activated protein kinase (MAPK) signalling pathway. The oncogenic mutations in the kinase region of BRAF gene result in constitutive activation of the MAPK signalling pathway, leading to increased cell proliferation, resistance to apoptosis and tumor progression. The most common of all activating BRAF mutations leads to a substitution of valine (V) to glutamic acid (E) at the position 600 of the amino acid sequence. The �BRAF �V600E mutation is an important predictive and prognostic biomarker. The BRAF V600E mutation is detected in approximately 8% of all solid tumours, including 45% of papillary thyroid carcinomas, 40-60% of melanomas, 5-15% of colorectal adenocarcinomas, 35% of serous low grade and borderline ovarian carcinomas, 1-3% of non-small cell lung cancers, and 5-7% of cholangiocarcinomas. �Furthermore, the BRAF V600E mutation is found in 100% of hairy cell leukaemia, �54% Erdheim-Chester disease, 38% of Langerhans cell histocytoses �and 60% of pleomorphic xanthoastrocytomas. �

Product Properties & Targets

Antibody Type
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus. Cytoplasm. Cell membrane.
Gene Name
Positive Control
A431, Jurkat and HeLa cells. Melanoma or Colon Carcinoma.
Recombinant fragment of human HMG20B protein (exact sequence is proprietary)
Alternate Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related, BRCA2-associated factor 35, HMG box-containing protein 20B, HMG domain-containing protein 2, HMG domain-containing protein HMGX2, Sox-like transcriptional factor, Structural DNA-binding protein BRAF35, BRAF25; BRAF35; BRCA2-associated factor 35; High Mobility Group Protein 20B (HM20B); HMG box-containing protein 20B; HMG domain-containing protein HMGX2; HMG20B; SMARCE1-related protein; SMARCE1r; Sox-like transcriptional factor; SOXL

Database Links

Entrez Gene ID

Additional Information

Chromosome Location
Mol. Weight of Antigen
35kDa (predicted)


  • Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.

Key References

  • Dvorak, Katerina; et al. Pathology, 2014, 46(6): 509-517.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.


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