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Recombinant Tenascin C (Stromal Marker for Epithelial Malignancy) Antibody [TNC/9734R]

In Stock
Catalog Number Formulation Size Price
3371-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3371-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3371-RBM8-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Tenascin C Antibody in Western Blot (WB)

Tenascin C Antibody in Western Blot (WB)

Western blot analysis of Human Kidney tissue lysate using Tenascin C Recombinant Rabbit Monoclonal Antibody (TNC/9734R).

Tenascin C Antibody in Western Blot (WB)

Tenascin C Antibody in Western Blot (WB)

Western blot analysis of Kidney tissue lysate of different species using Tenascin C Recombinant Rabbit Monoclonal Antibody (TNC/9734R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Extracellular matrix, Extracellular space, Secreted
Gene Name
TNC
Positive Control
Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell and lung carcinomas. Staining of normal fibroblasts serves as internal positive control.
Immunogen
Recombinant fragment (around aa275-400) of the human Tenascin protein (exact sequence is proprietary)
Alternate Names
Tenascin, 150-225|DFNA56|GMEM|GP|HXB|JI|TN|TN-C

Database Links

Entrez Gene ID
3371
SwissProt
P24821

Additional Information

Clone
TNC/9734R
Chromosome Location
9q33.1
Mol. Weight of Antigen
240.85kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All TNC Antibodies

Functions

  • Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed:19884327).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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