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Recombinant Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody [MSVA-605M]

Additional information

Catalog Number: 3852-MSM11
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3852-MSM11-P0
Purified Ab with BSA and Azide
20ug
$259.00
3852-MSM11-P1
Purified Ab with BSA and Azide
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
KRT5 Antibody in Numerous Tissues (IHC)

KRT5 Antibody in Numerous Tissues (IHC)

Keratin, type II cytoskeletal 5 Mouse Recombinant Monoclonal Antibody (MSVA-605M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:100-1:200 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-605M
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT5
Positive Control
Tonsil: A moderate to strong cytoplasmic KRT5 staining should be seen in virtually all squamous epithelial cells. Prostate: A strong cytoplasmic staining should be present in the majority of basal cells.
Immunogen
Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein (exact sequence is proprietary)
Expression System
CHO
Storage & Stability
Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Key References
  • Ordonez NG. Human Pathology. 2007; 38:1 16.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3852
SwissProt
P13647
Chromosome Location
12q13.13
Mol. Weight of Antigen
58kDa
Alternate Names
Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, 58kDa Cytokeratin; DDD1; Epidermolysis Bullosa Simplex 2 (EBS2); Keratin 5; Keratin, Type II Cytoskeletal 5; Keratin-5; Type-II Cytoskeletal 5; Type-II keratin Kb5

Summary

This Mab recognizes a protein of 58kDa, which is identified as Cytokeratin 5 (KRT5). This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, yoepithelial, and mesothelium.Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express.Anti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5.

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