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Recombinant Androgen Receptor (Marker of Androgen Dependence) Antibody [MSVA-367R]

Additional information

Catalog Number: 367-RBM29
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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AR Antibody in Numerous Tissues (IHC)

AR Antibody in Numerous Tissues (IHC)

Androgen receptor Rabbit Recombinant Monoclonal Antibody (MSVA-367R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
In Stock
Catalog Number Formulation Availability Size Price
367-RBM29-P0
Purified Ab with BSA and Azide
Trial Size
In Stock
20ug
$324.50
Trial Size Available In-Stock
367-RBM29-P1
Purified Ab with BSA and Azide
In Stock
100ug
$654.50
In-Stock
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:100-1:200 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-367R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
AR
Positive Control
Kidney: An at least weak to moderate nuclear AR immunostaining should be seen in a subset of kidney tubuli and of glomerular cells.
Immunogen
Synthetic peptide corresponding to residues (within aa1-100) of Androgen Receptor
Expression System
CHO
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
  • Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
  • Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
367
SwissProt
P10275
Chromosome Location
Xq12
Mol. Weight of Antigen
110kDa
Alternate Names
Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AIS; Dihydrotestosterone receptor (DHTR); HUMARA; HYSP1; Kennedy disease (KD); Nuclear receptor subfamily 3 group C member 4 (NR3C4); SMAX1; Spinal and bulbar muscular atrophy (SBMA); Testicular Feminization (TFM)

Summary

Androgen Receptor is a member of the superfamily of ligand responsive transcription regulators. The androgen receptor functions in the nucleus where it is believed to act as a transcriptional regulator mediating the action of male sex hormones.The androgen receptor has wide distribution and can be demonstrated by immunohistochemistry in several tissues including prostate, skin, and oral mucosa. Androgen receptor has been reported in a diverse range of human tumors including osteosarcoma, and in prostatic carcinoma androgen receptor expression may be of clinical relevance.Androgen Receptor is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains.

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