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Western blot analysis of human brain tissue lysate using Calbindin Recombinant Mouse Monoclonal Antibody (rCALB1/9805).
Formalin-fixed, paraffin-embedded human kidney stained with Calbindin Recombinant Mouse Monoclonal Antibody (rCALB1/9805). Inset: PBS instead of primary antibody; secondary only negative control.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It functions as a buffer for cytoplasmic calcium and is found in the brain, kidney, gut and pancreatic islets. In normal brain, calbindin has been identified in medium sized neurons of the neuropil of the matrix compartment of the striatum, the woolly fiber arrangements of the globus pallidus and the fiber structures of the pars reticula of the substantia nigra. A proportion of dendritic cells within the light zone of germinal centers have also been noted to be positive for calbindin.
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