These antibodies perform exceptionally well in the intended applications and have undergone stringent testing and additional validation to ensure monospecificity.
| Catalog Number: | 6285-RBM42 |
|---|---|
| Reactivity: | |
| Gene: | |
| Host: |
Trial Size Available
Carrier-Free Available
Western blot analysis of human brain tissue lysate using S100B Rabbit Recombinant Monoclonal Antibody (S100B/12587R).
Formalin-fixed, paraffin-embedded human brain stained with S100B Rabbit Recombinant Monoclonal Antibody (S100B/12587R). Inset: PBS instead of primary antibody; secondary only negative control.
SDS-PAGE Analysis of Purified S100B Recombinant Rabbit Monoclonal Antibody (S100B/12587R). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of human brain tissue lysate using S100B Rabbit Recombinant Monoclonal Antibody (S100B/12587R).
Western blot analysis of Brain tissue lysates of different species using S100B Rabbit Recombinant Monoclonal Antibody (S100B/12587R).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
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