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Recombinant Chromogranin A / CHGA (Neuroendocrine Marker) Antibody [CHGA/8929R]

Additional information

Catalog Number: 1113-RBM28
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1113-RBM28-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1113-RBM28-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1113-RBM28-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Chromogranin A / CHGA (Neuroendocrine Marker) Antibody

Formalin-fixed, paraffin-embedded human pancreas stained with Chromogranin A Recombinant Rabbit Monoclonal Antibody (CHGA/8929R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Chromogranin A / CHGA (Neuroendocrine Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified CHGA Recombinant Rabbit Monoclonal Antibody (CHGA/8929R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
CHGA/8929R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm.
Gene Name
CHGA
Positive Control
bowel|or pheochromocytoma.|pancreas|PC12 cells. Adrenal gland|thyroid
Immunogen
Recombinant full-length human Chromogranin A protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Strongly inhibits glucose induced insulin release from the pancreas.
  • Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172, PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).
  • Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Key References
  • Schmid, K.W., et al. 1993. Histopathology 22: 527-533.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1113
SwissProt
P10645
Chromosome Location
14q32.12
Mol. Weight of Antigen
51kDa
Alternate Names
Chromogranin-A | Vasostatin-1 | Vasostatin-2 | EA-92 | ES-43 | Pancreastatin | SS-18 | WA-8 | WE-14 | LF-19 | Catestatin | AL-11 | GV-19 | GR-44 | ER-37 | GE-25 | Serpinin-RRG | Serpinin | p-Glu serpinin precursor, Pituitary secretory protein I, Vasostatin I, Vasostatin II, SL21, Beta-Granin; CGA; CHGA; Chromogranin A Parathyroid Secretory Protein 1; ER-37; Pancreastatin; Parastatin; Pituitary Secretory Protein I; SP-I; Vasostatin I or II

Summary

Chromogranin A (CGA) is an acidic protein which is reported to be widely expressed in neural tissues and in secretory granules of human endocrine cells e.g. parathyroid gland, adrenal medulla, anterior pituitary gland, islet cells of the pancreas and C cells of the thyroid.GA is found in the large majority of epithelial NE tumors: pituitary adenomas, parathyroid and pancreatic islet cell tumors, medullary thyroid carcinoma, and carcinoid tumors.Together with synaptophysin, CGA is the most important marker for the identification of NE and neuronal tumors and NE/neuronal differentiation in other tumors.

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