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Recombinant CD35 / CR1 (Follicular Dendritic Cell Marker) Antibody [CR1/8244R]

Additional information

Catalog Number: 1378-RBM20
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1378-RBM20-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1378-RBM20-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1378-RBM20-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD35 / CR1 (Follicular Dendritic Cell Marker) Antibody

SDS-PAGE Analysis of Purified CD35 Recombinant Rabbit Monoclonal Antibody (CR1/8244R). Confirmation of Purity and Integrity of Antibody.

Recombinant CD35 / CR1 (Follicular Dendritic Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with CD35 Recombinant Rabbit Monoclonal Antibody (CR1/8244R). Inset: PBS instead of primary antibody; secondary only negative control.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
CR1/8244R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell surface.
Gene Name
CR1
Positive Control
Follicular dendritic cells (FDC) in tonsil.
Immunogen
Recombinant fragment (around aa600-900) of human CR1 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed:2963069). Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed:2963069). Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d, g, and of C4b to C4c and C4d (PubMed:2972794, PubMed:8175757). Also plays a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed:25742728).
  • (Microbial infection) Acts as a receptor for Epstein-Barr virus.
Key References
  • Hogg N, et. al. European Journal of Immunology, 1984, 14:236-43.
  • Biddle DA et al. Mod Pathol. 2002 Jan;15(1):50-8
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1378
SwissProt
P17927 Human
Chromosome Location
1q32.2
Mol. Weight of Antigen
224kDa
Alternate Names
Complement receptor type 1, C3b/C4b receptor, C3 binding protein, C3b/C4b receptor, C3BR, C4BR, Complement Component (3b/4b) receptor 1 including Knops blood group system, Complement receptor type 1, KN, Knops blood group antigen

Summary

CD35, complement receptor 1, is a cell membrane-bound, monomeric glycoprotein on numerous cell types including erythrocytes, leukocytes, glomerular podocytes, and follicular dendritic cells. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. CD35 antigen is found on erythrocytes, B cells, a subset of T cells, monocytes, as well as eosinophils, and neutrophils. Anti-CD35 is considered a mature B-cell marker which labels follicular dendritic reticulum cells and tumors derived from such cells such as follicular dendritic cell tumor/sarcoma.

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