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Myeloid-Associated Differentiation Marker (MYADM) Antibody [MYADM/972]

Additional information

Catalog Number: 91663-MSM2
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
91663-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
91663-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
91663-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Myeloid-Associated Differentiation Marker (MYADM) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with MYDAM Monoclonal Antibody (MYADM/972).

Myeloid-Associated Differentiation Marker (MYADM) Antibody - Image 2

SDS-PAGE Analysis of Purified MYDAM Monoclonal Antibody (MYADM/972). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Macaque Monkey
Clone
MYADM/972
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Membrane
Gene Name
MYADM
Positive Control
HL60 cells. Tonsil or lymph node.
Immunogen
Recombinant human MYADM protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Key References
  • Membrane protein hMYADM preferentially expressed in myeloid cells is up-regulated during differentiation of stem cells and myeloid leukemia cells. Wang Q, et al. Life Sci, 2007 Jan 9. PMID 17097684
  • Cloning of human myeloid-associated differentiation marker (MYADM) gene whose expression was up-regulated in NB4 cells induced by all-trans retinoic acid. Cui W, et al. Mol Biol Rep, 2001. PMID 12075932
  • MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration. Aranda JF, et al. Mol Biol Cell, 2011 Apr 15. PMID 21325632
  • MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression. Aranda JF, et al. Mol Biol Cell, 2013 Feb. PMID 23264465
  • Isolation of MYADM, a novel hematopoietic-associated marker gene expressed in multi-potent progenitor cells and up-regulated during myeloid differentiation. Pettersson M, et al. J Leukoc Biol, 2000 Mar. PMID 10733104
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
91663
SwissProt
Q96S97
Chromosome Location
19q13.42
Mol. Weight of Antigen
Unknown
Alternate Names
Myeloid-associated differentiation marker, Protein SB135, MYADM; myeloid associated differentiation marker; Myeloid upregulated protein; Protein SB135

Summary

Recognizes a myeloid associated differentiation antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of human and monkey myeloid cells. This MAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.

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