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Recombinant PAX5 / BSAP (Early B-Cell Marker) Antibody [rPAX5/2060]

Additional information

Catalog Number: 5079-MSM4
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
5079-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5079-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5079-MSM4-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
PAX5 Antibody in Western Blot (WB)

PAX5 Antibody in Western Blot (WB)

Western Blot Analysis of human Raji cell lysate using PAX5 Mouse Recombinant Monoclonal Antibody (rPAX5/2060).

PAX5 Antibody in SDS-PAGE

PAX5 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Paired box protein Pax-5 Recombinant Mouse Monoclonal Antibody (rPAX5/2060). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rPAX5/2060
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
PAX5
Positive Control
Daudi or Ramos cells. Tonsil, Lymph Nodeor Colon Carcinoma., Spleen. Raji
Immunogen
Recombinant full-length human PAX5 protein
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Additional information

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Gene:

Host:

Reactivity:

, , ,

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH2/1426).
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VIEW ALL ANTIBODIES

Other Information

Functions
  • Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed:10811620, PubMed:27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed:10811620, PubMed:27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed:32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed:32612238)., (Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription (PubMed:31941781). Also participates in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity (PubMed:23678172).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
5079
SwissProt
Q02548
Chromosome Location
9p13.2
Mol. Weight of Antigen
42.15kDa
Alternate Names
Paired box protein Pax-5, ALL3|BSAP|PAX-5

Summary

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. The PAX5 gene is a member of thepaired box (PAX)family of transcription factors. The central feature of this gene family is a novel, highly conservedDNA-binding domain, known as thepaired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute toneoplastic transformation. The PAX5 gene encodes theB-celllineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development andspermatogenesis.

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