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Recombinant Androgen Receptor (Marker of Androgen Dependence) Antibody [DHTR/4445R]

These antibodies perform exceptionally well in the intended applications and have undergone stringent testing and additional validation to ensure monospecificity.

The MAbMan recommends these antibodies for repeatable and reliable results.

Additional information

Catalog Number: 367-RBM5
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
367-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
367-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
367-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Androgen Receptor (Marker of Androgen Dependence) Antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Androgen Receptor Recombinant Rabbit Antibody (DHTR/4445R). HIER: Tris/EDTA pH9.0; 95°C/45min. 1°CAb: 2ug/ml in PBS:30min. 2°CAb: HRP-Poly:30min. DAB:5min.

Recombinant Androgen Receptor (Marker of Androgen Dependence) Antibody - Image 2

Formalin-fixed, paraffin-embedded human placenta stained with Androgen Receptor Recombinant Rabbit Antibody (DHTR/4445R). Negative tissue control. PBS used instead of 1°C Ab. HIER and all other steps of protocol identical.

Androgen Receptor Antibody in SDS-PAGE

Androgen Receptor Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified DHTR Recombinant Rabbit Monoclonal Antibody (DHTR/4445R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
DHTR/4445R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
AR
Positive Control
Breast, LNCap cells. Human testis, prostate or prostate carcinoma.
Immunogen
Synthetic peptide corresponding to residues (within aa1-100) of Androgen Receptor
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
  • Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
  • Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
367
SwissProt
P10275
Chromosome Location
Xq12
Mol. Weight of Antigen
110kDa
Alternate Names
Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AIS; Dihydrotestosterone receptor (DHTR); HUMARA; HYSP1; Kennedy disease (KD); Nuclear receptor subfamily 3 group C member 4 (NR3C4); SMAX1; Spinal and bulbar muscular atrophy (SBMA); Testicular Feminization (TFM)

Summary

Androgen Receptor is a member of the superfamily of ligand responsive transcription regulators. The androgen receptor functions in the nucleus where it is believed to act as a transcriptional regulator mediating the action of male sex hormones.The androgen receptor has wide distribution and can be demonstrated by immunohistochemistry in several tissues including prostate, skin, and oral mucosa. Androgen receptor has been reported in a diverse range of human tumors including osteosarcoma, and in prostatic carcinoma androgen receptor expression may be of clinical relevance.Androgen Receptor is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains.

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