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CD32 (Fc Gamma RIIa) Antibody [7.3]

Additional information

Catalog Number: 2212-MSM2
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
CD32 (Fc Gamma RIIa) Antibody

SDS-PAGE Analysis of Purified CD32 Mouse Monoclonal Antibody (7.30). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • Functional Studies
  • IF
In Stock
Catalog Number Formulation Availability Size Price
2212-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
Trial Size
In Stock
20ug
$259.00
Trial Size Available In-Stock
2212-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
In Stock
100ug
$559.00
In-Stock
2212-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Carrier-Free
In Stock
100ug
$559.00
Carrier-Free Available In-Stock
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence
Species Reactivity
Human
Clone
7.3
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
FCGR2A
Positive Control
K-562 cells. Lymph node, tonsil and placenta.
Immunogen
K562 and FcgRII+L cells
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Key References
  • Ierino et al., J. Immunol, 150: 17941803 (1993).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2212
SwissProt
P12318
Chromosome Location
1q23
Mol. Weight of Antigen
40kDa
Alternate Names
Low affinity immunoglobulin gamma Fc region receptor II-a, CDw32, Fc-gamma RII-a, CD32A; Fc fragment of IgG low affinity IIa receptor; Fc gamma RIIa; Fc-gamma RII-a; Fc-gamma-RIIa; FCGR2A; FcgRII; Fcr-2; FcRII-a; IGFR2; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a; Ly-17; Ly-m20; LyM-1; Lymphocyte antigen 17

Summary

This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

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