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ZNF81 / Zinc finger protein 81 (Transcription Factor) Antibody [PCRP-ZNF81-2C7]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
347344-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
347344-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
347344-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Flow cytometric analysis of PFA-fixed HeLa cells. ZNF81 Mouse Monoclonal Antibody (PCRP-ZNF81-2C7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow cytometric analysis of PFA-fixed HeLa cells. ZNF81 Mouse Monoclonal Antibody (PCRP-ZNF81-2C7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ZNF81 Mouse Monoclonal Antibody (PCRP-ZNF81-2C7).

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ZNF81 Mouse Monoclonal Antibody (PCRP-ZNF81-2C7).

followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

SDS-PAGE Analysis of Purified ZNF81 Mouse Monoclonal Antibody (PCRP-ZNF81-2C7). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified ZNF81 Mouse Monoclonal Antibody (PCRP-ZNF81-2C7). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a krueppel-type DNAbinding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF81, also known as HFZ20 or MRX45, is a transcriptional regulator belonging to the krueppel C2H2-type zincfinger protein family. It localizes to the nucleus and contains 12 C2H2-type zinc fingers and one KRAB domain. Mutations in the gene encoding ZNF81 are implicated in nonsyndromic X-linked mental retardation (XLMR).

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG2b /
Cellular Localization
Nucleus
Gene Name
ZNF81
Positive Control
HeLa or HepG2 cells.
Immunogen
Recombinant full-length human ZNF81 protein
Alternate Names
Zinc finger protein 81, HFZ20, HFZ20; Mental Retardation, X-linked 45 (MRX45)

Database Links

Entrez Gene ID
347344
SwissProt
P51508

Additional Information

Clone
PCRP-ZNF81-2C7
Chromosome Location
Xp11.23
Mol. Weight of Antigen
75.9kDa

Related Antibodies

View All ZNF81 Antibodies

Functions

  • May be involved in transcriptional regulation.

Key References

  • Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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