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Formalin-fixed, paraffin-embedded human skeletal muscle stained with ZNF804A Mouse Monoclonal Antibody (ZNF804A/8945). Inset: PBS instead of primary antibody; secondary only negative control.
SDS-PAGE Analysis of Purified ZNF804A Mouse Monoclonal Antibody (ZNF804A/8945). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
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Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF804A (zinc-finger protein 804A) is a 1,209 amino acid protein that contains one C2H2-type zinc finger. ZNF804A interacts with Ataxin-1, a protein that is involved in RNA metabolism. The gene encoding ZNF804A maps to human chromosome 2, which consists of 237 million bases and encodes over 1,400 genes, making up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2.
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