Tubulin-alpha (Microtubule Marker) Antibody [TUBA/3038]

In Stock
Catalog Number Formulation Size Price
7277-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
7277-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
7277-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. This MAb recognizes an epitope of alpha-tubulin. The alpha-tubulin is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants – tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Cytoskeleton
Gene Name
Positive Control
Human prostate, lung or colon tissues (IHC).
Immunogen
Recombinant fragment (aa 1-115) of human Tubulin Alpha protein (exact sequence is proprietary)
Alternate Names
Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, Alpha-tubulin 1; Hum a tub1; Hum a tub2; Testis-specific alpha-tubulin; TUBA1; TUBA1A; Tuba4a; Tubulin alpha-1 chain; Tubulin H2-alpha; Tubulin, alpha 1 (testis specific); Tubulin, alpha 4a

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
TUBA/3038
Chromosome Location
2q35
Mol. Weight of Antigen
55kDa

Functions

  • Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Key References

  • Draber, P., et al. (1989) J. Cell Sci. 92, 519-528.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Tubulin-alpha (Microtubule Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK