Formalin-fixed, paraffin-embedded human Melanoma stained with Topo I, MT Mouse Monoclonal Antibody (TOP1MT/568).

Formalin-fixed, paraffin-embedded human Melanoma stained with Topo I, MT Mouse Monoclonal Antibody (TOP1MT/568)

SDS-PAGE Analysis Purified Topo I, MT Mouse Monoclonal Antibody (TOP1MT/568). Confirmation of Purity and Integrity of Antibody.

Printable Datasheet

Topoisomerase (DNA) I, Mitochondrial (TOP1MT) [TOP1MT/568]

Name: Topoisomerase (DNA) I, Mitochondrial (TOP1MT)
SKU: 116447-MSM2
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Product Properties & Targets

Host

Mouse

Applications

Immunohistochemistry

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

Mitochondrion

Light Chain

Kappa

Gene Name

TOP1MT

Positive Control

A431 cells. Heart, brain or fetal liver., Skeletal muscle

Immunogen

Recombinant full-length human TOP1MT protein

Alternate Names

DNA topoisomerase I, mitochondrial, DNA topoisomerase I; TOP1MT; Topoisomerase I mitochondrial; Type IB Topoisomerase

Database Links

Entrez Gene ID

116447

Unigene

528574

SwissProt

Q969P6

Additional Information

Clone

TOP1MT/568

Human Gene Symbol

TOP1MT

Chromosome Location

8q24.3

Mol. Weight of Antigen

70kDa

Functions

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Zhang, H., et al. 2001. Proc. Natl. Acad. Sci. USA 98: 10608-10613.
Zhang, H., et al. 2007. Biochimie 89: 474-481.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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