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Th-Pok / ZBTB7B Antibody [PCRP-ZBTB7B-1B6]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
51043-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
51043-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
51043-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Flow Cytometric Analysis of PFA-fixed HeLa cells. Th-Pok / ZBTB7B Mouse Monoclonal Antibody (PCRP-ZBTB7B-1B6) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Flow Cytometric Analysis of PFA-fixed HeLa cells. Th-Pok / ZBTB7B Mouse Monoclonal Antibody (PCRP-ZBTB7B-1B6) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunofluorescence Analysis of PFA-fixed HeLa cells using Th-Pok / ZBTB7B Mouse Monoclonal Antibody (PCRP-ZBTB7B-1B6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Immunofluorescence Analysis of PFA-fixed HeLa cells using Th-Pok / ZBTB7B Mouse Monoclonal Antibody (PCRP-ZBTB7B-1B6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Analysis of Protein Array containing more than 19,000 full-length human proteins using Th-Pok / ZBTB7B Mouse Monoclonal Antibody (PCRP-ZBTB7B-1B6). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Analysis of Protein Array containing more than 19,000 full-length human proteins using Th-Pok / ZBTB7B Mouse Monoclonal Antibody (PCRP-ZBTB7B-1B6). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

SDS-PAGE Analysis. Purified Th-Pok / ZBTB7B Mouse Monoclonal Antibody (PCRP-ZBTB7B-1B6). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis. Purified Th-Pok / ZBTB7B Mouse Monoclonal Antibody (PCRP-ZBTB7B-1B6). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

TH-POK (T-helper-inducing POZ/Kr ppel-like factor), also known as zinc finger protein 67 (ZFP67), zinc finger and BTB domain-containing protein 7B or Kr ppel-related zinc finger protein cKrox, is a 539 amino acid protein that contains one BTB (POZ) domain and four C2H2-type zinc fingers. Localized to the nucleus, TH-POK functions primarily as a key regulator of lineage commitment of immature T-cell precursors. Specifically, the presence of TH-POK directs positively selected thymocytes to the CD4 lineage, whereas its absence causes default development to the CD8 lineage. TH-POK also functions as a transcriptional repressor of various other genes, such as COL1A1, COL1A2 and Fibronectin.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Research Areas
Nuclear Marker
Isotype / Light Chain
IgG2b /
Cellular Localization
Nucleus
Gene Name
ZBTB7B
Positive Control
HeLa or HepG2 cells.
Immunogen
Recombinant full-length human ZBTB7B protein
Alternate Names
Zinc finger and BTB domain-containing protein 7B, Krueppel-related zinc finger protein cKrox, T-helper-inducing POZ/Krueppel-like factor, Zinc finger and BTB domain-containing protein 15, Zinc finger protein 67 homolog, Zinc finger protein 857B, Zinc finger protein Th-POK, cKrox; hcKrox; T helper inducing POZ/Kruppel like factor; Th-POK; ThPOK; ZBTB15; ZBTB7B; ZFP67; Zinc finger and BTB domain containing 15; Zinc finger and BTB domain containing 7B; Zinc finger protein 67 homolog; Zinc finger protein 857B; Zinc finger protein ThPOK; ZNF857B

Database Links

Entrez Gene ID
51043
SwissProt
O15156

Additional Information

Clone
PCRP-ZBTB7B-1B6
Chromosome Location
1q21.3
Mol. Weight of Antigen
58/80kDa

Related Antibodies

View All ZBTB7B Antibodies

Functions

  • Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Exerts distinct biological functions in the mammary epithelial cells and T cells in a tissue-specific manner. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Cross-antagonism between ZBTB7B and CBF complexes are determinative to CD4 versus CD8 cell fate decision. Suppresses RUNX3 expression and imposes CD4+ lineage fate by inducing the SOCS suppressors of cytokine signaling. induces, as a transcriptional activator, SOCS genes expression which represses RUNX3 expression and promotes the CD4+ lineage fate. During CD4 lineage commitment, associates with multiple sites at the CD8 locus, acting as a negative regulator of the CD8 promoter and enhancers by epigenetic silencing through the recruitment of class II histone deacetylases, such as HDAC4 and HDAC5, to these loci. Regulates the development of IL17-producing CD1d-restricted naural killer (NK) T cells. Also functions as an important metabolic regulator in the lactating mammary glands. Critical feed-forward regulator of insulin signaling in mammary gland lactation, directly regulates expression of insulin receptor substrate-1 (IRS-1) and insulin-induced Akt-mTOR-SREBP signaling (By similarity). Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes (PubMed:9370309). Potent driver of brown fat development, thermogenesis and cold-induced beige fat formation. Recruits the brown fat lncRNA 1 (Blnc1):HNRNPU ribonucleoprotein complex to activate thermogenic gene expression in brown and beige adipocytes (By similarity).

Key References

  • He, X., et al. 2005. The zinc finger transcription factor TH-POK regulates CD4 versus CD8 T-cell lineage commitment. Nature 433: 826-833.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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