Formalin-fixed, paraffin-embedded human liposarcoma stained with STAT6 Recombinant Rabbit Monoclonal Antibody (STAT6/7163R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with STAT6 Recombinant Rabbit Monoclonal Antibody (STAT6/7163R).

Formalin-fixed, paraffin-embedded human cerebellum stained with STAT6 Recombinant Rabbit Monoclonal Antibody (STAT6/7163R). Inset: PBS instead of primary antibody; secondary only negative control.

Printable Datasheet

Recombinant STAT6 (Solitary Fibrous Tumor Marker) [STAT6/7163R]

Name: STAT6 (Solitary Fibrous Tumor Marker)
SKU: 6778-RBM3
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

STAT6 is a transcription factor in the Jak/STAT signal transduction pathway responsible for mediating IL-4 immune signaling. STAT6 was recently suggested to be a reliable marker to distinguish solitary fibrous tumors from other soft tissue neoplasms. Gene fusions are common in solitary fibrous tumors. Recent next generation sequencing studies demonstrated the presence of a NAB2-STAT6 fusion, formed by an intrachromosomal inversion fusing two neighboring genes on chromosome 12q13, in 55-100% of solitary fibrous tumors, regardless of tumor morphology or anatomical site. By immunohistochemistry, nuclear STAT6 expression can discriminate solitary fibrous tumors from its morphological mimics in the meninges, including meningioma, glioblastoma, gliosarcoma, haemangioblastoma, schwannoma and haemangioma. A recent study by Cheah, et al. using the rabbit monoclonal STAT6 antibody (Clone YE361) observed expression in all solitary fibrous tumors (54/54) tested, regardless of histology, anatomical site or CD34 status. Morphological mimics of solitary fibrous tumors were negative, demonstrating 100% specificity.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cytoplasm, Nucleus

Light Chain

kappa

Gene Name

STAT6

Positive Control

HeLa or HepG2 cells. Human kidney.

Immunogen

Recombinant full-length human STAT6 protein

Alternate Names

Signal transducer and activator of transcription 6, IL-4 Stat, IL4 STAT; Interleukin 4 Induced Transcription Factor IL4 STAT; Signal transducer and activator of transcription 6; STAT interleukin4 induced; STAT6; Transcription factor IL 4 STAT

Database Links

Entrez Gene ID

6778

Unigene

524518

SwissProt

P42226

Additional Information

Clone

STAT6/7163R

Human Gene Symbol

STAT6

Chromosome Location

12q13.3

Mol. Weight of Antigen

119kDa

Functions

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Hou, J., et al. 1994. An interleukin-4-induced transcription factor: IL-4 Stat. Science 265: 1701-1706.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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