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RPS6KA5 / MSK1 Antibody [PCRP-RPS6KA5-1A8]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
9252-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
9252-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
9252-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Flow Cytometric Analysis of PFA-fixed HeLa cells. RPS6KA5 / MSK1 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of PFA-fixed HeLa cells. RPS6KA5 / MSK1 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescence Analysis of HeLa cells using RPS6KA5 / MSK1 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Immunofluorescence Analysis of HeLa cells using RPS6KA5 / MSK1 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Analysis of Protein Array containing more than 19,000 full-length human proteins using RPS6KA5 / MSK1 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Analysis of Protein Array containing more than 19,000 full-length human proteins using RPS6KA5 / MSK1 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

SDS-PAGE Analysis. Purified RPS6KA5 / MSK1 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis. Purified RPS6KA5 / MSK1 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. A related S6 kinase, p70 S6 kinase, functions to phosphorylate the S6 protein on ribosomal 40S subunits. p70 S6 kinase b shares high sequence homology with p70 S6 kinase, except in the carboxy terminus where it contains a proline-rich domain that may be involved in SH3 domain containing protein interactions. MSK1 (also designated RLPK) is related to Rsk and p70 S6 kinase family members and is thought to be structurally similar to Rsk family members, but it may be regulated by distinct mechanisms.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Research Areas
Developmental Biology, Immunology, Bladder Cancer, Cytokine Signaling, MAPK Signaling, Nuclear Marker, Signal Transduction
Isotype / Light Chain
IgG2b /
Cellular Localization
Cytoplasm, Nucleus
Gene Name
RPS6KA5
Positive Control
HeLa or JAR cells. Ubiquitous nuclear expression. Human prostate, tonsil or brain.
Immunogen
Recombinant full-length human RPS6KA5 protein
Alternate Names
Ribosomal protein S6 kinase alpha-5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, RSK-like protein kinase, 90 kDa ribosomal protein S6 kinase 5; EC 2.7.11.1; Mitogen and stress activated protein kinase 1; MSPK1; Nuclear Mitogen And Stress Activated Protein Kinase 1; Ribosomal protein S6 kinase 90kD polypeptide 5; Ribosomal Protein S6 Kinase Alpha 5; RLPK; RPS6KA5; RSK Like Protein Kinase; RSKL; S6K alpha 5

Database Links

Entrez Gene ID
9252
SwissProt
O75582

Additional Information

Clone
PCRP-RPS6KA5-1A8
Chromosome Location
14q32.11
Mol. Weight of Antigen
~90kDa

Related Antibodies

View All RPS6KA5 Antibodies

Functions

  • Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes (PubMed:11909979, PubMed:12569367, PubMed:12763138, PubMed:9687510, PubMed:18511904, PubMed:9873047). Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin (PubMed:11909979, PubMed:9873047). Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression (PubMed:12628924, PubMed:18511904). In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress (PubMed:12628924). In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential (PubMed:12763138). Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation (PubMed:12569367). Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A (PubMed:15010469). Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN (PubMed:12773393). May also phosphorylate 'Ser-28' of histone H3 (PubMed:12773393). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14) (PubMed:12773393). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines (By similarity). Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity). Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury (By similarity). Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity (PubMed:25851810).

Key References

  • Alcorta, D.A., et al. 1989. Sequence and expression of chicken and mouse Rsk: homologs of Xenopus laevis ribosomal S6 kinase. Mol. Cell. Biol. 9: 3850-3859.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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