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Recombinant Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody [VIL1/7378R]

Additional information

Catalog Number: 7429-RBM8
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
7429-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7429-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7429-RBM8-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody

Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Villin Recombinant Rabbit Monoclonal Antibody (VIL1/7378R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Villin Recombinant Rabbit Monoclonal Antibody (VIL1/7378R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Villin Antibody in Western Blot (WB)

Villin Antibody in Western Blot (WB)

Western Blot Analysis of A549 cell lysate using Villin Recombinant Rabbit Monoclonal Antibody (VIL1/7378R).

VIL1 Antibody in SDS-PAGE

VIL1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Villin-1 Recombinant Rabbit Monoclonal Antibody (VIL1/7378R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
VIL1/7378R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell projection, Cytoplasm, Cytoskeleton, Filopodium, Filopodium tip, Lamellipodium, Microvillus, Ruffle
Gene Name
VIL1
Positive Control
A549|HepG2 or HCT116 cells. Human colon or rectum.
Immunogen
Recombinant fragment (around aa681-693) of the human Villin protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7429
SwissProt
P09327
Chromosome Location
2q35
Mol. Weight of Antigen
92.69kDa
Alternate Names
Villin-1, D2S1471|VIL

Summary

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin.Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

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