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Recombinant Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker) Antibody [TYRP1/1564R]

In Stock
Catalog Number Formulation Size Price
7306-RBM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7306-RBM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7306-RBM4-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker) Antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with TYRP1 Rabbit Recombinant Monoclonal Antibody (TYRP1/1564R) using AEC Chromogen.

TYRP1 Antibody in SDS-PAGE

TYRP1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified 5,6-dihydroxyindole-2-carboxylic acid oxidase Recombinant Rabbit Monoclonal Antibody (TYRP1/1564R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It reacts with a 75kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi.Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Melanosome membrane
Gene Name
TYRP1
Positive Control
SK-MEL-19, SK-MEL-23, SK-MEL-30, SK-MEL-37 cells. Melanoma.
Immunogen
Recombinant human TYRP1 protein
Alternate Names
5,6-dihydroxyindole-2-carboxylic acid oxidase, CAS2|CATB|GP75|OCA3|TRP|TRP1|TYRP|b-PROTEIN

Database Links

Entrez Gene ID
7306
SwissProt
P17643

Additional Information

Clone
TYRP1/1564R
Chromosome Location
9p23
Mol. Weight of Antigen
60.72kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All TYRP1 Antibodies

Functions

  • Plays a role in melanin biosynthesis (PubMed:16704458, PubMed:22556244, PubMed:23504663). Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu(2+) ions, but not in the presence of Zn(2+) (PubMed:28661582). May regulate or influence the type of melanin synthesized (PubMed:16704458, PubMed:22556244). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (By similarity).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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