Recombinant Tubulin beta 3 / TUBB3 (Neuronal Stem Cell Marker) Antibody [rTUBB3/7406]

In Stock
Catalog Number Formulation Size Price
10381-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
10381-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
10381-MSM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. �In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Product Properties & Targets

Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell projection, Cytoplasm, Cytoskeleton, Filopodium, Growth cone, Lamellipodium
Gene Name
Positive Control
brain or testis. PC3 cells.
Immunogen
Recombinant fragment (around aa437-450) of the human TUBB3 (Tubulin beta 3 class III) protein (exact sequence is proprietary)
Alternate Names
Tubulin beta-3 chain, CDCBM|CDCBM1|CFEOM3|CFEOM3A|FEOM3|TUBB4|beta-4

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
rTUBB3/7406
Chromosome Location
16q24.3
Mol. Weight of Antigen
50.43kDa
Expression System
HEK293 Cells
Purification
Protein G

Functions

  • Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed:34996871, PubMed:38305685, PubMed:38609661). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871, PubMed:38305685, PubMed:38609661). Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871, PubMed:38609661). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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