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Recombinant TCF4 (Transcription Factor 4) Antibody [TCF4/16911R]

In Stock
Catalog Number Formulation Size Price
6925-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6925-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6925-RBM5-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
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Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5′-CANNTG-3′). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5′-ACANNTGT-3′ or 5′-CCANNTGG-3′.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus
Gene Name
TCF4
Positive Control
Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung
Immunogen
Recombinant fragment (around aa365-671) of the human TCF4 protein (exact sequence is proprietary)
Alternate Names
Transcription factor 4, CDG2T|E2-2|FCD2|FECD3|ITF-2|ITF2|PTHS|SEF-2|SEF2|SEF2-1|SEF2-1A|SEF2-1B|SEF2-1D|TCF-4|bHLHb19

Database Links

Entrez Gene ID
6925
SwissProt
P15884

Additional Information

Clone
TCF4/16911R
Chromosome Location
18q21.2
Mol. Weight of Antigen
71.31kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All TCF4 Antibodies

Functions

  • Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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