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Recombinant Spectrin beta I (SPTB) Antibody [SPTBN2/2894R]

Additional information

Catalog Number: 6712-RBM7
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6712-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6712-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6712-RBM7-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Spectrin beta I (SPTB) Antibody

Western Blot Analysis of HeLa cell lysates using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

Recombinant Spectrin beta I (SPTB) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Pancreatic Cancer stained with Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

Recombinant Spectrin beta I (SPTB) Antibody - Image 3

Formalin-fixed, paraffin-embedded human Pancreatic Cancer stained with Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

Recombinant Spectrin beta I (SPTB) Antibody - Image 4

Western Blot Analysis of Human PANC-1 cell lysate using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

Recombinant Spectrin beta I (SPTB) Antibody - Image 5

Flow Cytometric Analysis of HeLa cells using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

SPTBN2 Antibody in SDS-PAGE

SPTBN2 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Spectrin beta chain, non-erythrocytic 2 Rabbit Monoclonal Antibody (SPTBN2/2894R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
SPTBN2/2894R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell cortex, Cytoplasm, Cytoskeleton
Gene Name
SPTBN2
Positive Control
HeLa cells. Pancreas or Liver.
Immunogen
Recombinant full-length human Spectrin Beta 3 protein
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Probably plays an important role in neuronal membrane skeleton.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6712
SwissProt
O15020
Chromosome Location
11q13.2
Mol. Weight of Antigen
271.32kDa
Alternate Names
Spectrin beta chain, non-erythrocytic 2, GTRAP41|SCA5|SCAR14

Summary

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

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