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Recombinant PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody [UCHL1/7076R]

In Stock
Catalog Number Formulation Size Price
7345-RBM16-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7345-RBM16-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7345-RBM16-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody

ormalin-fixed, paraffin-embedded human cerebellum stained with Pgp9.5 Rabbit Recombinant Monoclonal Antibody (UCHL1/7076R). Inset: PBS instead of primary antibody; secondary only negative control

Recombinant PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified Pgp9.5 Rabbit Recombinant Monoclonal Antibody (UCHL1/7076R). Confirmation of Purity and Integrity of Antibody.

PGP9.5 / UchL1 Antibody in Western Blot (WB)

PGP9.5 / UchL1 Antibody in Western Blot (WB)

Western Blot Analysis of human brain tissue lysate using PGP9.5 / UchL1 Recombinant Rabbit Monoclonal Antibody (UCHL1/7076R).

PGP9.5 / UchL1 Antibody in Western Blot (WB)

PGP9.5 / UchL1 Antibody in Western Blot (WB)

Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain and Guinea pig Brain using PGP9.5 / UchL1 Recombinant Rabbit Monoclonal Antibody (UCHL1/7076R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson’s disease.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Guinea Pig, Hamster, Human, Mouse, Rat
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Endoplasmic reticulum membrane
Gene Name
UCHL1
Positive Control
Brain or cerebellum.
Immunogen
Recombinant fragment (around aa185-214) of the human pgp9.5 (UCHL-1) protein (exact sequence is proprietary)
Alternate Names
Ubiquitin carboxyl-terminal hydrolase isozyme L1, HEL-117|HEL-S-53|NDGOA|PARK5|PGP 9.5|PGP9.5|PGP95|SPG79|SPG79A|UCHL-1|Uch-L1

Database Links

Entrez Gene ID
7345
SwissProt
P09936

Additional Information

Clone
UCHL1/7076R
Chromosome Location
4p13
Mol. Weight of Antigen
24.82kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All UCHL1 Antibodies

Functions

  • Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed:22212137, PubMed:23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed:22212137, PubMed:25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed:12408865, PubMed:8639624, PubMed:9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed:22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed:25615526).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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