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Recombinant PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody [rPCNA/8858]

Additional information

Catalog Number: 5111-MSM11
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
5111-MSM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5111-MSM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5111-MSM11-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with PCNA Recombinant Mouse Monoclonal Antibody (rPCNA/8858) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with PCNA Recombinant Mouse Monoclonal Antibody (rPCNA/8858) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

PCNA Antibody in SDS-PAGE

PCNA Antibody in SDS-PAGE

SDS-PAGE Analysis Purified PCNA Recommbinant Mouse Monoclonal Antibody (rPCNA/8859). Confirmation of Purity and Integrity of Antibody.

PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody in Western Blot (WB)

PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody in Western Blot (WB)

Western Blot Analysis of A-431, 293T and Hep G2 cell lysates using PCNA Recombinant Mouse Monoclonal Antibody (rPCNA/8858).

PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody in Western Blot (WB)

PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody in Western Blot (WB)

Western Blot Analysis of Human Intestine,Human Skin, A431, A549, HeLa, HEK293, K-562, Jurkat and Raw lysates using PCNA Recombinant Mouse Monoclonal Antibody (rPCNA/8858).

PCNA Antibody in Western Blot (WB)

PCNA Antibody in Western Blot (WB)

Western blot analysis of skin and intestine tissue lysates from human, mouse, rat, hamster, guinea pig and rabbit using PCNA Recombinant Mouse Monoclonal Antibody (rPCNA/8858).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rPCNA/8858
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
PCNA
Positive Control
Hep G2, U937 cells.|Tonsil or reactive lymph node. SKBr3, Human Intestine,Human Skin, A431, A549, HeLa, HEK293, K-562, Jurkat or Raw.
Immunogen
PCNA made in the protein A expression vector pR1T2T of rat origin.
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed:35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
5111
SwissProt
P12004
Chromosome Location
20p12.3
Mol. Weight of Antigen
28.77kDa
Alternate Names
Proliferating cell nuclear antigen, ATLD2

Summary

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

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