Printable Datasheet

Recombinant OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody [OCT4/13757R]

In Stock
Catalog Number Formulation Size Price
5460-RBM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5460-RBM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5460-RBM12-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Awaiting product image

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Transcription factor that binds to the octamer motif (5′-ATTTGCAT-3′). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
POU5F1
Positive Control
Expressed in developing brain
Immunogen
15-amino acid inter-domain linker region (cGPEPGWVDPRTWLSF) connecting the two DNA-binding subdomains POUs and POUh within the POU domain of the Oct4 (POU5F1) protein
Alternate Names
POU domain, class 5, transcription factor 1, OCT3|OCT4|OCT4Borf1|OTF-3|OTF3|OTF4|Oct-3|Oct-4|Oct3/4

Database Links

Entrez Gene ID
5460
SwissProt
Q01860

Additional Information

Clone
OCT4/13757R
Chromosome Location
6p21.33
Mol. Weight of Antigen
38.57kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All POU5F1 Antibodies

Functions

  • Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales