Contact Us

Recombinant MSH6 (DNA Mismatch Repair Protein) Antibody [MSVA-906R]

Additional information

Catalog Number: 2956-RBM25
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
MSH6 Antibody in Numerous Tissues (IHC)

MSH6 Antibody in Numerous Tissues (IHC)

DNA mismatch repair protein Msh6 Rabbit Recombinant Monoclonal Antibody (MSVA-906R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
In Stock
Catalog Number Formulation Availability Size Price
2956-RBM25-P0
Purified Ab with BSA and Azide
Trial Size
In Stock
20ug
$324.50
Trial Size Available In-Stock
2956-RBM25-P1
Purified Ab with BSA and Azide
In Stock
100ug
$654.50
In-Stock
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:75-1:150 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-906R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Chromosome, Nucleus
Gene Name
MSH6
Positive Control
Tonsil: Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining. A moderate to strong nuclear staining must be seen in the germinal centre B-cells.
Immunogen
Recombinant fragment (around aa 1-100) of human MSH6 protein (exact sequence is proprietary)
Expression System
HEK293
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

SDS-PAGE Analysis of Purified P-Cadherin Mouse Monoclonal Antibody (6A9). Confirmation of Integrity and Purity of Antibody.
0
  • Flow
  • IF
  • WB

P-Cadherin (CDH3) Antibody

Additional information

Catalog Number: 1001-MSM2
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Occludin Mouse Monoclonal Antibody (OCLN/2181).
4
  • IHC
  • WB

Occludin (Marker of Early Blood Brain Barrier Damage) Antibody

Additional information

Catalog Number: 100506658-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded Mouse Heart stained with N-Cadherin Mouse Monoclonal Antibody (CDH2/1573).
1
  • IHC

N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM3
Reactivity:

,

Gene:

Host:

Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4443). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
5
  • IHC
  • WB

MICA Antibody

Additional information

Catalog Number: 100507436-MSM3
Reactivity:

Gene:

Host:

Flow cytometric analysis of PFA-fixed HeLa cells. MEF2B Mouse Monoclonal Antibody (PCRP-MEF2B-2F9) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
2
  • Flow
  • IF
  • WB

MEF2B (Transcriptional Activator) Antibody

Additional information

Catalog Number: 100271849-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded Human Renal Cell Carcinoma Stained with KSP-Cadherin Rabbit Polyclonal Antibody.
0
  • IHC

Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-RBP3
Reactivity:

, ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).
7
  • ELISA
  • Flow
  • IHC
  • WB

Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-MSM1
Reactivity:

, , , ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH2/1426).
2
  • IHC
  • WB

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM4
Gene:

Host:

Reactivity:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP
  • >ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
Key References
  • Blackwell LJ., et al. J Biol Chem. 1998 Nov 27;273(48):32055-62.
  • Iaccarino I., et al. EMBO J. 1998 May 1;17(9):2677-86.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2956
SwissProt
P52701
Chromosome Location
2p16.3
Mol. Weight of Antigen
153kDa
Alternate Names
DNA mismatch repair protein Msh6, G/T mismatch-binding protein, MutS protein homolog 6, MutS-alpha 160 kDa subunit, DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; mutS (E. coli) homolog 6; MutS alpha 160kDa subunit; MutS-alpha 160kDa subunit; p160; Sperm associated protein

Summary

MSH6 is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H). It has been suggested that the deficiencies in DNA mismatch repair protein(s) can be seen in some malignancies such as hereditary nonpolyposis colorectal cancer (HNPCC) and endometrial cancer. MSH6 expressed in all proliferating cells participate in repair of base-base mismatch, that occur during DNA replication. Loss of MSH6 expression leads to an accumulation of DNA replication errors in the proliferating cells, particularly in areas of the genome with short repetitive nucleotide sequences, a phenomenon known as microsatellite instability (MSI). MSH6 always used as panel with MLH1, MSH2, PMS2, and may be useful to aid in identifying the most probable gene responsiblefor the MSI.

Worry-Free Guarantee

Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.

Learn More

Need Technical Help?

Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.

CONTACT US

Follow Us on LinkedIn

NeoBiotechnologies develops and manufactures highly specific, validated monoclonal antibodies for cancer research and diagnostics.

Phone

Whatsapp

Quick Links

Information

© 2026 NeoBiotechnologies.  All Rights Reserved.

Terms and Conditions Privacy Policy
Contact Us