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Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody [MGMT/8319R]

Additional information

Catalog Number: 4255-RBM10
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4255-RBM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4255-RBM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4255-RBM10-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody

SDS-PAGE Analysis of Purified MGMT Mouse Monoclonal Antibody (MGMT/8319R). Confirmation of Purity and Integrity of Antibody.

Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with MGMT Mouse Monoclonal Antibody (MGMT/8319R). Inset: PBS instead of primary antibody; secondary only negative control.

MGMT Antibody in Western Blot (WB)

MGMT Antibody in Western Blot (WB)

Western Blot Analysis of MCF-7 lysate using MGMT Rabbit Recombinant Monoclonal Antibody (MGMT/8319R).

MGMT Antibody in Western Blot (WB)

MGMT Antibody in Western Blot (WB)

Western Blot Analysis of MCF-7, LNCaP, Hep G2 and human liver lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8319R).

MGMT Antibody in Western Blot (WB)

MGMT Antibody in Western Blot (WB)

Western blot analysis of Human Liver, Mouse Liver, Rat Liver, Hamster Liver and Guinea pig Liver lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8319R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
MGMT/8319R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
MGMT
Positive Control
MCF-7, LNCaP, Hep G2 and human liver, Jurkat. Human tonsil or stomach (IHC).
Immunogen
Recombinant full-length human MGMT protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4255
SwissProt
P16455
Chromosome Location
10q26.3
Mol. Weight of Antigen
21.65kDa
Alternate Names
Methylated-DNA--protein-cysteine methyltransferase, -

Summary

Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross �links at the O6 � alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 �position of guanine in DNA (prior to cross �link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA �damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

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