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Recombinant MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody [MLANA/8108R]

Additional information

Catalog Number: 2315-RBM20
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2315-RBM20-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2315-RBM20-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2315-RBM20-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody

Formalin-fixed, paraffin-embedded human skin stained with MART-1 Recombinant Rabbit Monoclonal Antibody (MLANA/8108R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

MART-1 Antibody in Western Blot (WB)

MART-1 Antibody in Western Blot (WB)

Western Blot Analysis of Colo-38 lysate using MART-1 Rabbit Recombinant Monoclonal Antibody (MLANA/8108R).

MART-1 Antibody in SDS-PAGE

MART-1 Antibody in SDS-PAGE

SDS-PAGE Analysis Purified MART1 Mouse Monoclonal Antibody (MLANA/8108R). Confirmation of Purity and Integrity of Antibody.

MART-1 Antibody in Western Blot (WB)

MART-1 Antibody in Western Blot (WB)

Western Blot Analysis of Colo-38 lysate using MART-1 Rabbit Recombinant Monoclonal Antibody (MLANA/8108R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
MLANA/8108R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Endoplasmic reticulum membrane, Golgi apparatus, Melanosome, trans-Golgi network membrane
Gene Name
MLANA
Positive Control
Human skin or melanoma. Colo-38 cells
Immunogen
Recombinant full-length human MART-1 protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2315
SwissProt
Q16655
Chromosome Location
9p24.1
Mol. Weight of Antigen
13.16kDa
Alternate Names
Melanoma antigen recognized by T-cells 1, MART-1|MART1

Summary

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

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