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Recombinant Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody [MSVA-516R]

Additional information

Catalog Number: 1014-RBM20
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1014-RBM20-P0
Purified Ab with BSA and Azide
20ug
$434.50
1014-RBM20-P1
Purified Ab with BSA and Azide
100ug
$874.50
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CDH16 Antibody in Numerous Tissues (IHC)

CDH16 Antibody in Numerous Tissues (IHC)

Cadherin-16 Rabbit Recombinant Monoclonal Antibody (MSVA-516R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:100-1:200 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-516R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
CDH16
Positive Control
Kidney: A strong staining should be seen in distal tubuli and collecting ducts while the staining is at least moderate in proximal tubuli.
Immunogen
Recombinant human full-length CDH16 protein
Expression System
HEK293
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Key References
  • Shen, S.S., et al. 2005. Kidney-specific cadherin, a specific marker for the distal portion of the nephron and related renal neoplasms. Mod Pathol. 18: 933-40.
  • Mazal, P.R., et al. 2005. Expression of kidney-specific cadherin distinguishes chromophobe renal cell carcinoma from renal oncocytoma. Hum Pathol. 36: 22-28.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1014
SwissProt
O75309
Chromosome Location
16q22.1
Mol. Weight of Antigen
130kDa
Alternate Names
Cadherin-16, Kidney-specific cadherin, Cadherin-16 (CDH16); Kidney-specific cadherin; Ksp-cadherin antibody

Summary

This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

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