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Recombinant HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Antibody [HSPD1/8406R]

Additional information

Catalog Number: 3329-RBM19
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3329-RBM19-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3329-RBM19-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3329-RBM19-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Antibody

Formalin-fixed, paraffin-embedded human kidney stained with HSP60 Rabbit Recombinant Monoclonal Antibody (HSPD1/8406R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified HSP60 Rabbit Recombinant Monoclonal Antibody (HSPD1/8406R). Confirmation of Purity and Integrity of Antibody.

HSP60 Antibody in Western Blot (WB)

HSP60 Antibody in Western Blot (WB)

Western blot analysis of Human Brain, Human Kidney, Human Pancreas, HeLa, HEK293, K-562 and Jurkat tissue lysates using HSP60 Recombinant Rabbit Monoclonal Antibody (HSPD1/8406R).

HSP60 Antibody in Western Blot (WB)

HSP60 Antibody in Western Blot (WB)

Western blot analysis of Brain, Kidney and Pancreas tissue lysates of different species using HSP60 Recombinant Rabbit Monoclonal Antibody (HSPD1/8406R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
HSPD1/8406R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Mitochondrion matrix
Gene Name
HSPD1
Positive Control
Human Brain, Human Kidney, Human Pancreas, HeLa, HEK293, K-562, Jurkat, Hep2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.
Immunogen
Recombinant full-length human HSP60 protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:11422376, PubMed:1346131). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3329
SwissProt
P10809
Chromosome Location
2q33.1
Mol. Weight of Antigen
61.05kDa
Alternate Names
60 kDa heat shock protein, mitochondrial, CPN60|GROEL|HLD4|HSP-60|HSP60|HSP65|HuCHA60|SPG13

Summary

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone HSPD1/8406R, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.

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