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Recombinant Growth Hormone (Pituitary Marker) Antibody [GH/8215R]

In Stock
Catalog Number Formulation Size Price
2688-RBM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2688-RBM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2688-RBM11-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Growth Hormone (Pituitary Marker) Antibody

Formalin-fixed, paraffin-embedded human pituitary stained with Growth Hormone Recombinant Rabbit Monoclonal Antibody (GH/8215R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant Growth Hormone (Pituitary Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human placenta stained with Growth Hormone Recombinant Rabbit Monoclonal Antibody (GH/8215R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant Growth Hormone (Pituitary Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified Growth Hormone Recombinant Rabbit Monoclonal Antibody (GH/8215R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Pituitary growth hormone (GH) plays a crucial role in stimulating and controlling the growth, metabolism and differentiation of many mammalian cell types by modulating the synthesis of multiple mRNA species. These effects are mediated by the binding of GH to its membrane-bound receptor, GHR, and involve a phosphorylation cascade that results in the modulation of numerous signaling pathways. GH is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Anti-GH is a useful marker in classification of pituitary tumors and the study of pituitary disease (acromegaly).

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Secreted
Gene Name
GH1
Positive Control
Pituitary cells. Human pituitary tissue (IHC).
Immunogen
Recombinant fragment (around aa58-187) of the human Growth Hormone (GH) protein (exact sequence is proprietary)
Alternate Names
Somatotropin, GH|GH-N|GHB5|GHN|IGHD1A|IGHD1B|IGHD2|hGH|hGH-N

Database Links

Entrez Gene ID
2688
SwissProt
P01241

Additional Information

Clone
GH/8215R
Chromosome Location
17q23.3
Mol. Weight of Antigen
24.85kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All GH1 Antibodies

Functions

  • Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

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