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Recombinant GP2 (Glycoprotein 2) / ZAP75 Antibody [GP2/3134R]

Additional information

Catalog Number: 2813-RBM8
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2813-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2813-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2813-RBM8-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant GP2 (Glycoprotein 2) / ZAP75 Antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with GP2 Recombinant Rabbit Monoclonal Antibody (GP2/3134R).

Recombinant GP2 (Glycoprotein 2) / ZAP75 Antibody - Image 2

SDS-PAGE Analysis of Purified GP2 Recombinant Rabbit Monoclonal Antibody (GP2/3134R). Confirmation of Purity and Integrity of Antibody.

GP2 Antibody in Western Blot (WB)

GP2 Antibody in Western Blot (WB)

Western Blot Analysis of Human Pancreas tissue lysates using GP2 Recombinant Rabbit Monoclonal Antibody (GP2/3134R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
GP2/3134R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Endosome, Membrane raft, Secreted, Zymogen granule membrane
Gene Name
GP2
Positive Control
PANC-1 cells. Pancreas.
Immunogen
Recombinant fragment (around aa35-179) of the human GP2 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Functions as an intestinal M-cell transcytotic receptor specific for type-I-piliated bacteria that participates in the mucosal immune response toward these bacteria. At the apical membrane of M-cells it binds fimH, a protein of the bacteria type I pilus tip. Internalizes bound bacteria, like E.coli and S.typhimurium, from the lumen of the intestine and delivers them, through M-cells, to the underlying organized lymphoid follicles where they are captured by antigen-presenting dendritic cells to elicit a mucosal immune response.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2813
SwissProt
P55259
Chromosome Location
16p12.3
Mol. Weight of Antigen
59.48kDa
Alternate Names
Pancreatic secretory granule membrane major glycoprotein GP2, ZAP75

Summary

GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

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