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Formalin-fixed, paraffin-embedded human breast carcinoma stained with Glutathione S-transferase pi 1 Recombinant Rabbit Monoclonal Antibody (GSTP1/12956R). Inset: PBS instead of primary antibody; secondary only negative control.
SDS-PAGE Analysis of Purified Glutathione S-transferase pi 1 Recombinant Rabbit Monoclonal Antibody (GSTP1/12956R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Rabbit Brain, Human Heart, Mouse Heart, Rat Heart, Hamster Heart, Guinea pig Heart, Rabbit Heart, Human Lung, Mouse Lung, Rat Lung, Hamster Lung, Guinea pig Lung and Rabbit Lung tissue lysates using GSTP1 Recombinant Rabbit Monoclonal Antibody (GSTP1/12956R).
Western Blot validation of anti-GSTP1 antibody. GSTP1 protein expression was compared in wild-type (WT) and GSTP1 shRNA Knockdown (KD) HeLa cells (30ug of total protein). Loading control is shown below.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Glutathione S-transferases (GSTs) function in the metabolic detoxification of various environmental carcinogens and lipid hydroperoxides. In response to oxidative stress, upregulation of the GST family member GSTP1 occurs, consistent with this function. Furthermore, the GSTP1 gene is subject to CpG island hyper-methylation, a state that correlates with human prostatic carcinogenesis. GSTP1 gene hyper-methylation can be detected in urine, ejaculate and plasma from men with prostate cancer, potentially making GSTP1 a useful biomarker for prostate cancer screening.
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