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Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody [KRT7/1499R]

Additional information

Catalog Number: 3855-RBM9
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3855-RBM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3855-RBM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3855-RBM9-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Oviduct stained with Cytokeratin 7 Rabbit Monoclonal Antibody (KRT7/1499R).

Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 2

Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Rabbit Monoclonal Antibody (KRT7/1499R).

Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 3

Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytokeratin 7 Rabbit Recombinant MAb (KRT7/1499R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red)

KRT7 Antibody in SDS-PAGE

KRT7 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Keratin, type II cytoskeletal 7 Recombinant Rabbit Monoclonal Antibody (KRT7/1499R). Confirmation of Purity and Integrity of Antibody.

Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 5

SDS-PAGE Analysis of Purified Cytokeratin 7 Rabbit Recombinant Monoclonal Antibody (KRT7/1499R).

Cytokeratin 7 Antibody in Western Blot (WB)

Cytokeratin 7 Antibody in Western Blot (WB)

Western blot analysis of human lung tissue lysates using Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/1499R).

KRT7 Antibody in Western Blot (WB)

KRT7 Antibody in Western Blot (WB)

Western blot analysis of human lung tissue lysates using KRT7 Recombinant Rabbit Monoclonal Antibody (KRT7/1499R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
KRT7/1499R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT7
Positive Control
cervix or breast., HeLa cells. Carcinoma of ovary, Lung
Immunogen
Recombinant full-length human Cytokeratin 7 protein
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Additional information

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Gene:

Host:

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3855
SwissProt
P08729
Chromosome Location
12q13.13
Mol. Weight of Antigen
51.39kDa
Alternate Names
Keratin, type II cytoskeletal 7, CK7|K2C7|K7|SCL

Summary

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

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