| Catalog Number: | 1152-RBM16 |
|---|---|
| Gene: | |
| Host: | |
| Reactivity: | Guinea Pig, Hamster, Human, Mouse, Rat |
Trial Size Available
Carrier-Free Available

Formalin-fixed, paraffin-embedded human brain stained with CKBB Recombinant Rabbit Monoclonal Antibody (CKBB/8608R). Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis of Purified CKBB Recombinant Rabbit Monoclonal Antibody (CKBB/8608R). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of HEK293 lysate using CKBB Recombinant Rabbit Monoclonal Antibody (CKBB/8608R).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.
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