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Recombinant Complement Component C9 (CCC9) (Early Myocardial Infarction Marker) Antibody [r10A6]

Additional information

Catalog Number: 735-MSM3
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
735-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
735-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
735-MSM3-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Component C9 Antibody in Immunohistochemistry (IHC (P))

Component C9 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with Complement Component C9 Recombinant Mouse Monoclonal Antibody (r10A6). Inset: PBS instead of primary antibody; secondary only negative control.

Component C9 Antibody in SDS-PAGE

Component C9 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Complement Component C9 Recombinant Mouse Monoclonal Antibody (r10A6). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
r10A6
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Secreted, Target cell membrane
Gene Name
C9
Positive Control
Human liver, placenta, myocardium or rejected renal transplant tissue.
Immunogen
Recombinant full-length human Complement Component C9 (CCC9) protein
Expression System
CHO
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Pore-forming component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed:22832194, PubMed:26841837, PubMed:26841934, PubMed:27052168, PubMed:30552328, PubMed:6177822, PubMed:9212048, PubMed:9634479). The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways (PubMed:9212048, PubMed:9634479). The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:9212048, PubMed:9634479). Constitutes the pore-forming subunit of the MAC complex: during MAC assembly, C9 associates with the C5b8 intermediate complex, and polymerizes to complete the pore (PubMed:26841934, PubMed:30111885, PubMed:30552328, PubMed:34752492, PubMed:4055801, PubMed:6177822).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
735
SwissProt
P02748
Chromosome Location
5p13.1
Mol. Weight of Antigen
63.17kDa
Alternate Names
Complement component C9, ARMD15|C9D

Summary

Complement component C9 binds to the C5b-8 complex as the final protein of the membrane attack complex. After binding, it undergoes a conformational change and inserts itself into the cell membrane, forming transmembrane channels. Complement component C9 acts in a similar way
to perforin, a pore forming protein found in cytotoxic T cells. Male and female reproductive tissues express and synthesize complement components, binding proteins and receptors, although the implications of this are unclear.

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