Recombinant Collagen IV Antibody [COL4/4241R]

In Stock
Catalog Number Formulation Size Price
1282-RBM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1282-RBM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1282-RBM1-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Collagen Type IV is a major component of the basement membrane and plays an important role in cell adhesion, migration, differentiation and growth. Collagen Type IV express at the basement membranes in a variety of tissues including kidney, muscle, lymph nodes, lung, tendon and spleen. Collagen Type IV has been shown to be useful in differentiating microinvasive from in situ ductal carcinomas of the breast. Other Collagen Type IV studies include use in pancreatic adenocarcinoma and chronic pancreatitis, nephrosclerosis and other kidney diseases, oral squamous cell carcinoma, laryngeal cancers, ovarian cancers and cervical cancers.

Product Properties & Targets

Host
Rabbit
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Basement membrane, Extracellular matrix, Extracellular space, Secreted
Gene Name
Positive Control
Human placenta or tonsil.
Immunogen
Recombinant fragment (around aa1-1669) of the human Collagen IV protein (exact sequence is proprietary)
Alternate Names
Collagen alpha-1(IV) chain, BSVD|BSVD1|COL4A1s|PADMAL|RATOR

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
COL4/4241R
Chromosome Location
13q34
Mol. Weight of Antigen
160.61kDa
Expression System
CHO Cells
Purification
Protein A

Functions

  • Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen., Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK