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Recombinant Collagen IV alpha 5 chain / COL4A5 Antibody [COL4A5/9396R]

In Stock
Catalog Number Formulation Size Price
1287-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1287-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1287-RBM3-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Collagen IV alpha 5 chain / COL4A5 Antibody

Formalin-fixed, paraffin-embedded human pancreas stained with COL4A5 Recombinant Rabbit Monoclonal Antibody (COL4A5/9396R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant Collagen IV alpha 5 chain / COL4A5 Antibody - Image 2

SDS-PAGE Analysis of Purified COL4A5 Recombinant Rabbit Monoclonal (COL4A5/9396R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Type IV collagen is the major structural component of the glomerular basement membrane (GBM), forming a hexagonal network together with lamins, proteoglycans, and nestin. Deletions of the N-terminal regions of COL4A5 and COL4A6 were found in Xq22.3 chromosome deletion syndrome and were located in an end-to-end manner. This results in a phenotype characteristic of diffuse leiomyomatosis and Alport syndrome (DL-ATS).

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Basement membrane, Extracellular matrix, Extracellular space, Secreted
Gene Name
COL4A5
Positive Control
Human placenta or tonsil.
Immunogen
Recombinant fragment (around aa1461-1685) of the human Collagen IV alpha 5 chain protein (exact sequence is proprietary)
Alternate Names
Collagen alpha-5(IV) chain, ASLN|ATS|ATS1|CA54

Database Links

Entrez Gene ID
1287
SwissProt
P29400

Additional Information

Clone
COL4A5/9396R
Chromosome Location
Xq22.3
Mol. Weight of Antigen
161.04kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All COL4A5 Antibodies

Functions

  • Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

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