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Recombinant CD50 / ICAM3 Antibody [rICAM3/1019]

Additional information

Catalog Number: 3385-MSM7
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3385-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3385-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3385-MSM7-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD50 / ICAM3 Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD50 Recombinant Mouse Monoclonal Antibody (rICAM3/1019).

Recombinant CD50 / ICAM3 Antibody - Image 2

SDS-PAGE Analysis of Purified CD50 Recombinant Mouse Monoclonal Antibody (rICAM3/1019). Confirmation of Purity and Integrity of Antibody.

CD50 Antibody in Western Blot (WB)

CD50 Antibody in Western Blot (WB)

Western Blot Analysis of Jurkat cell lysate using CD50 Monoclonal Antibody (rICAM3/1019).

  • IHC
  • WB
  • Flow
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Clone
rICAM3/1019
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Membrane
Gene Name
ICAM3
Positive Control
HL-60 or THP-1 cells. Human lymph node or tonsil. Jurkat.
Immunogen
Recombinant fragment (around aa1-547) of the human CD50 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3385
SwissProt
P32942
Chromosome Location
19p13.2
Mol. Weight of Antigen
59.54kDa
Alternate Names
Intercellular adhesion molecule 3, CD50|CDW50|ICAM-R

Summary

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.

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