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Recombinant CD48 (Pan Leukocyte Marker) Antibody [rCD48/8834]

In Stock
Catalog Number Formulation Size Price
962-MSM15-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
962-MSM15-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
962-MSM15-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD48 (Pan Leukocyte Marker) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with CD48 Recombinant Mouse Monoclonal Antibody (rCD48/8834). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant CD48 (Pan Leukocyte Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified CD48 Recombinant Mouse Monoclonal Antibody (rCD48/8834). Confirmation of Purity and Integrity of Antibody.

CD48 Antibody in Western Blot (WB)

CD48 Antibody in Western Blot (WB)

Western blot analysis of Human Spleen and Raji lysates using CD48 Monospecific Recombinant Mouse Monoclonal Antibody (rCD48/8834).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Membrane raft, Secreted
Gene Name
CD48
Positive Control
Raji cells. Human lymph node or tonsil. Spleen.
Immunogen
Recombinant fragment (around aa59-178) of the human CD48 protein (exact sequence is proprietary)
Alternate Names
CD48 antigen, BCM1|BLAST|BLAST1|MEM-102|SLAMF2|hCD48|mCD48

Database Links

Entrez Gene ID
962
SwissProt
P09326

Additional Information

Clone
rCD48/8834
Chromosome Location
1q23.3
Mol. Weight of Antigen
27.68kDa
Expression System
HEK293 Cells
Purification
Protein G

Related Antibodies

View All CD48 Antibodies

Functions

  • Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including CD244/2B4 or CD2 to regulate immune cell function and activation (PubMed:12007789, PubMed:19494291, PubMed:27249817, PubMed:9841922). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed:19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed:12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed:27249817).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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