Contact Us

Recombinant CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Antibody [CD27/9812R]

Additional information

Catalog Number: 939-RBM23
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
In Stock
Catalog Number Formulation Size Price
939-RBM23-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
939-RBM23-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
939-RBM23-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Awaiting product image
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
CD27/9812R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
CD27
Positive Control
Found in most T-lymphocytes
Immunogen
Prokaryotic recombinant protein corresponding to the complete CD27 molecule excluding the signal sequence.
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH16/1071).
7
  • Flow
  • IHC
  • WB

Recombinant Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-MSM4
Reactivity:

, , , ,

Gene:

Host:

SDS-PAGE Analysis of Purified P-Cadherin Mouse Monoclonal Antibody (12H6). Confirmation of Integrity and Purity of Antibody.
0
  • Flow
  • IF
  • WB

P-Cadherin (CDH3) Antibody

Additional information

Catalog Number: 1001-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2183).
4
  • IHC
  • WB

Occludin (OCLN) (Tight Junctions Marker) Antibody

Additional information

Catalog Number: 100506658-MSM3
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4442). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
5
  • IHC
  • WB

MICA Antibody

Additional information

Catalog Number: 100507436-MSM2
Reactivity:

, , , ,

Gene:

Host:

Flow cytometric analysis of PFA-fixed HeLa cells. MEF2B Mouse Monoclonal Antibody (PCRP-MEF2B-2F9) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
2
  • Flow
  • IF
  • WB

MEF2B (Transcriptional Activator) Antibody

Additional information

Catalog Number: 100271849-MSM1
Reactivity:

Gene:

Host:

CDH16 Antibody in Immunohistochemistry (IHC (P))
6
  • ELISA
  • IHC
  • WB

Ksp-Cadherin / CDH16 (Renal Cell Marker) Antibody

Additional information

Catalog Number: 1014-MSM5
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2616).
3
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM6
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/3874R).
3
  • IHC
  • WB

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-RBM5
Gene:

Host:

Reactivity:

, , ,

VIEW ALL ANTIBODIES

Other Information

Functions
  • Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells (PubMed:28011863). The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed:28011863). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (PubMed:38354704). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (By similarity). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (By similarity).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
939
SwissProt
P26842
Chromosome Location
12p13.31
Mol. Weight of Antigen
29.14kDa
Alternate Names
CD27 antigen, S152|S152. LPFS2|T14|TNFRSF7|Tp55

Summary

Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells (PubMed:28011863). The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed:28011863). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (PubMed:38354704). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (By similarity). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (By similarity).

Worry-Free Guarantee

Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.

Learn More

Need Technical Help?

Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.

CONTACT US

Follow Us on LinkedIn

NeoBiotechnologies develops and manufactures highly specific, validated monoclonal antibodies for cancer research and diagnostics.

Phone

Whatsapp

Quick Links

Information

© 2026 NeoBiotechnologies.  All Rights Reserved.

Terms and Conditions Privacy Policy
Contact Us